709. Characterization of Nanoparticles in Lentiviral Vector Preparations

Lentiviral Vector

A retroviral vector system based on the human immunodeficiency virus (HIV) was developed that, in contrast to a murine leukemia virus-based counterpart, transduced heterologous sequences into HeLa cells and rat fibroblasts blocked in the cell cycle, as well as into human primary macrophages. Additionally, the HIV vector could mediate stable in vivo gene transfer into terminally differentiated n...

Expression of Recombinant Alpha-1 Antitrypsin in CHO and COS-7 Cell Lines Using Lentiviral Vector

In this study, in order to facilitate and accelerate the production of eukaryotic protein alpha 1-antitrypsin (AAT) with correct post-translational modifications, a protein production system based on the transduction of CHO and COS-7 cells using lentiviral vectors was developed. Human AAT cDNA was cloned into a replication-defective lentiviral vector. The transgene AAT-Jred chimer was transferr...

Gene Regulatable Lentiviral Vector System

Comparison of lentiviral vector titration methods

BACKGROUND Lentiviral vectors are efficient vehicles for stable gene transfer in dividing and non-dividing cells. Several improvements in vector design to increase biosafety and transgene expression, have led to the approval of these vectors for use in clinical studies. Methods are required to analyze the quality of lentiviral vector production, the efficiency of gene transfer and the extent of...

A Modified lentiviral vector construction system

Objective: To develop a lentiviral vector system, which can be used to co-express multiple genes of interest, such as siRNAs cassettes, reporter gene and resistant gene simultaneously and to facilitate to titrate lentiviral vector stock with TCID50 method. Method: Synthesis new multiple cloning sites(MCS)to replace the original MCS of pLKO-1-puro and produced an intermediate plasmid pLKO-1-puro...